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This thesis presents the development of an instrument-free thermal cycler for polymerase chain reaction (PCR) aimed at low-resource settings lacking access to electricity. Two prototypes were designed and tested, with the second prototype achieving stable temperature maintenance for over 2576 seconds, significantly improving reaction efficiency. The findings suggest future work should focus on creating a steady temperature gradient to enhance DNA amplification through natural convection currents.

Author

gabriel.heredia

Language

EN