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This document discusses DNA amplification using polymerase chain reaction (PCR). It begins with an introduction to PCR, describing how it was originally developed in the 1980s and is now commonly used to detect and isolate DNA sequences of interest. The principle of PCR is then explained, noting that it allows specific DNA sequences to be copied from genomic DNA using thermostable DNA polymerase. The essential components of a PCR reaction are listed, including template DNA, primers, DNA polymerase, dNTPs, d

Author

daloo dmdm

Language

EN