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Hepatitis B virus replication in primary macaque hepatocytes: Crossing the species barrier toward a new small primate model by Julie Lucifora; Isabelle E. Vincent; Pascale Berthillon; Tatiana Dupinay; Maud Michelet; Ulrike Protzer; Fabien Zoulim; David Durantel; Christian Trepo; Isabelle Chemin is a Medicine article available to read on EtoBox.

What is Hepatitis B virus replication in primary macaque hepatocytes: Crossing the species barrier toward a new small primate model about?

The development of new anti-hepatitis B virus (HBV) therapies, especially immunotherapeutic approaches, has been limited by the lack of a primate model more accessible than chimpanzees. We have previously demonstrated that sylvanus and cynomolgus macaques are susceptible to in vivo HBV infection after intrahepatic HBV DNA inoculation. In this study, we evaluated the susceptibility of primary macaque hepatocytes (PMHs) to HBV infection with a highly efficient HBV genome-mediated transfer system via a recombinant baculovirus (Bac-HBV). Freshly prepared PMHs, isolated from macaque liver tissue by collagenase perfusion, were transduced with Bac-HBV, and intermediates of replication were followed for 9 days post-transduction. Evidence of HBV replication (hepatitis B surface antigen secretion, viral DNA, RNA, and covalently closed circular DNA) was detected from day 1 to day 9 post-transduction. HBV markers were dose-dependent and still detectable at a multiplicity of infection of 10. Importantly, transduced PMHs secreted all typical forms of HBV particles, as evidenced by a cesium chloride gradient as well as transmission electron microscopy. Furthermore, the Toll-like receptor 9 (TLR9)

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Author

Julie Lucifora; Isabelle E. Vincent; Pascale Berthillon; Tatiana Dupinay; Maud Michelet; Ulrike Protzer; Fabien Zoulim; David Durantel; Christian Trepo; Isabelle Chemin

Publisher

Ovid Technologies (Wolters Kluwer Health)

Published

2010

Language

EN

Field

Medicine (Health Sciences)