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Methylation-specific digital karyotyping of HPV16E6E7-expressing human keratinocytes identifies novel methylation events in cervical carcinogenesis: DNA methylation by HPV16E6E7 by Renske DM Steenbergen; Maté Ongenaert; Suzanne Snellenberg; Geert Trooskens; Wendy F van der Meide; Deeksha Pandey; Noga Bloushtain-Qimron; Kornelia Polyak; Chris JLM Meijer; Peter JF Snijders; Wim Van Criekinge is a Medicine article available to read on EtoBox.

What is Methylation-specific digital karyotyping of HPV16E6E7-expressing human keratinocytes identifies novel methylation events in cervical carcinogenesis: DNA methylation by HPV16E6E7 about?

Transformation of epithelial cells by high-risk human papillomavirus (hrHPV) types can lead to anogenital carcinomas, particularly cervical cancer, and oropharyngeal cancers. This process is associated with DNA methylation alterations, often affecting tumour suppressor gene expression. This study aimed to comprehensively unravel genome-wide DNA methylation events linked to a transforming hrHPV-infection, which is driven by deregulated expression of the viral oncogenes E6 and E7 in dividing cells. Primary human keratinocytes transduced with HPV16E6E7 and their untransduced counterparts were subjected to methylation-specific digital karyotyping (MSDK) to screen for genome-wide DNA-methylation changes at different stages of HPV-induced transformation. Integration of the obtained methylation profiles with genome-wide gene expression patterns of cervical carcinomas identified 34 genes with increased methylation in HPV-transformed cells and reduced expression in cervical carcinomas. For 12 genes (CLIC3, CREB3L1, FAM19A4, LFNG, LHX1, MRC2, NKX2-8, NPTX-1, PHACTR3, PRDM14, SOST and TNFSF13) specific methylation in HPV-containing cell lines was confirmed by semi-quantitative methylation-spe

Who reads Methylation-specific digital karyotyping of HPV16E6E7-expressing human keratinocytes identifies novel methylation events in cervical carcinogenesis: DNA methylation by HPV16E6E7?

Author

Renske DM Steenbergen; Maté Ongenaert; Suzanne Snellenberg; Geert Trooskens; Wendy F van der Meide; Deeksha Pandey; Noga Bloushtain-Qimron; Kornelia Polyak; Chris JLM Meijer; Peter JF Snijders; Wim Van Criekinge

Publisher

Wiley

Published

2013

Language

EN

Field

Medicine (Health Sciences)