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Safety and Efficacy of the Immunosuppressive Agent 6-Tioguanine in Murine Model of Acute and Chronic Colitis by Miloslav Kverka; Pavel Rossmann; Helena Tlaskalova-Hogenova; Klara Klimesova; Bindia Jharap; Nanne K de Boer; Rene M Vos; Adriaan A van Bodegraven; Milan Lukas; Chris J Mulder is a Medicine article available to read on EtoBox.
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## Background Oral thiopurines are effective and widely used in treatment of inflammatory bowel disease (IBD) in humans, although their use is limited due the development of adverse events. Here, we examine the efficacy and toxicity of oral treatment with 6-tioguanine (6-TG) and azathioprine (AZA) in a murine model of IBD. ## Methods We induced acute or chronic colitis in BALB/c mice by one or four cycles of 3% dextran sulphate sodium (DSS), respectively. Mice were treated by daily gavages of various dosages of 6-tioguanine, azathioprine, or by phosphate buffered saline (PBS) starting the first day of DSS or after two cycles of DSS, respectively. We monitored the efficacy and toxicity by measuring the weight change and serum alanine aminotransferase (ALT) activity and by disease severity and histology, at the end of the experiment. Moreover, we measured cytokine production after colon fragment cultivation by enzyme-linked immunoabsorbent assay and numbers of apoptotic cells in the spleen by flow cytometry. ## Results 6-TG is effective in the treatment of acute DSS-induced colitis in a dose-dependent manner and 40 μg of 6-TG is significantly more effective in the treatment of acute
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- Author
- Miloslav Kverka; Pavel Rossmann; Helena Tlaskalova-Hogenova; Klara Klimesova; Bindia Jharap; Nanne K de Boer; Rene M Vos; Adriaan A van Bodegraven; Milan Lukas; Chris J Mulder
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-230X)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)