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Protocols For Nucleic Acid Analysis By Nonradioactive Probes Nonradioactive Probes by Peter G. Isaac (auth.), Peter G. Isaac (eds.) is a nonfiction available to read on EtoBox.

What is Protocols For Nucleic Acid Analysis By Nonradioactive Probes Nonradioactive Probes about?

This Book Provides An Exceptionally Useful Collection Of Tried And Tested Protocols For Nucleic Acid Analysis That Avoid The Use Of Radioisotopes. Southern (i.e., Dna) And Northern (i.e., Rna) Blotting Protocols Are Covered In Detail, From How To Isolate Good Quality Nucleic Acids From Plant And Animal Sources, To The Use Of The Widest Range Of Probe Systems (involving Digoxigenin, Biotin, Fluorescein, Horseradish Peroxidase Labeling, And Colorimetric And Chemiluminescent Detection). Other Techniques Treated In Detail Include Hybridization In Situ To Chromosomes And Transcribed Rna, Newer And Less Conventional Technologies, Such As Randomly Amplified Polymorphic Dna (rapd), Reverse Dot Blots, Bacterial Identification Using Magnetic Head And Dipstick Assays, And For The First Time In Detail, Nucleic Acid Sequence-based Amplification. About Nonradioactive Nucleic Acid Detection -- Isolation Of Dna From Plants -- Isolation Of High-molecular-weight Dna From Animal Cells -- Restriction Enzyme Digestion, Gel Electrophoresis And Vacuum Blotting Of Dna To Nylon Membranes -- Isolation Of Plant Rna -- Isolation Of Total And Polya Rna From Animal Cells -- Preparation Of Rna Gel Blots -- Prepa

Who reads Protocols For Nucleic Acid Analysis By Nonradioactive Probes Nonradioactive Probes?

Author

Peter G. Isaac (auth.), Peter G. Isaac (eds.)

Publisher

Humana Press : Imprint: Humana

Published

1993

Language

EN

ISBN

9780896032545

Category

nonfiction

Subjects

Science, Medical, Biology