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Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds by Peter C McKeown; Sylvia Laouielle-Duprat; Pjotr Prins; Philip Wolff; Marc W Schmid; Mark TA Donoghue; Antoine Fort; Dorota Duszynska; Aurélie Comte; Nga Thi Lao; Trevor J Wennblom; Geert Smant; Claudia Köhler; Ueli Grossniklaus; Charles Spillane is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds about?

## Background Epigenetic regulation of gene dosage by genomic imprinting of some autosomal genes facilitates normal reproductive development in both mammals and flowering plants. While many imprinted genes have been identified and intensively studied in mammals, smaller numbers have been characterized in flowering plants, mostly in __Arabidopsis thaliana__. Identification of additional imprinted loci in flowering plants by genome-wide screening for parent-of-origin specific uniparental expression in seed tissues will facilitate our understanding of the origins and functions of imprinted genes in flowering plants. ## Results cDNA-AFLP can detect allele-specific expression that is parent-of-origin dependent for expressed genes in which restriction site polymorphisms exist in the transcripts derived from each allele. Using a genome-wide cDNA-AFLP screen surveying allele-specific expression of 4500 transcript-derived fragments, we report the identification of 52 maternally expressed genes (MEGs) displaying parent-of-origin dependent expression patterns in Arabidopsis siliques containing F1 hybrid seeds (3, 4 and 5 days after pollination). We identified these MEGs by developing a bioinf

Who reads Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds?

Author

Peter C McKeown; Sylvia Laouielle-Duprat; Pjotr Prins; Philip Wolff; Marc W Schmid; Mark TA Donoghue; Antoine Fort; Dorota Duszynska; Aurélie Comte; Nga Thi Lao; Trevor J Wennblom; Geert Smant; Claudia Köhler; Ueli Grossniklaus; Charles Spillane

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2229)

Published

2011

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)