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Application of Cationic Probes for the Ultrastructural Localization of Proteoglycans in Basement Membranes by Toin H. M. S. M. van Kuppevelt; Jacques H. Veerkamp is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Application of Cationic Probes for the Ultrastructural Localization of Proteoglycans in Basement Membranes about?

The application of cationic probes for the ultrastructural detection of proteoglycans in basement membranes is reviewed. Proteoglycans are highly negatively charged macromolecules due to their glycosaminoglycan side chains. The interaction of cationic probes with proteoglycans is of an electrostatic nature. Methods are discussed to increase the specificity of probes for proteoglycans. The use of phthalocyanin-like dyes such as Cuprolinic blue, according to the critical electrolyte concentration method, results in a selective staining of proteoglycans. Enzymatic or chemical digestions, however, should be done to validate the proteoglycan nature of the dye-positive granules/filaments, and to establish the class of proteoglycan. The value of cationic probes in basement membrane research on development and pathology is discussed. The potential for deducing molecular information from the ultrastructural appearance of stained proteoglycans is indicated. o 1994 Wiley-Liss, Inc.

Who reads Application of Cationic Probes for the Ultrastructural Localization of Proteoglycans in Basement Membranes?

Author

Toin H. M. S. M. van Kuppevelt; Jacques H. Veerkamp

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1059-910X)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)