Opening book details…
Can I read Three-dimensional Spheroid Culture Induces Apical-basal Polarity and the Original Characteristics of Immortalized Human Renal Proximal Tubule Epithelial Cells on EtoBox?
Three-dimensional Spheroid Culture Induces Apical-basal Polarity and the Original Characteristics of Immortalized Human Renal Proximal Tubule Epithelial Cells by Ken Mizuguchi; Hiromasa Aoki; Mineyoshi Aoyama; Yohei Kawaguchi; Yuko Waguri-Nagaya; Nobuyuki Ohte; Kiyofumi Asai is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Three-dimensional Spheroid Culture Induces Apical-basal Polarity and the Original Characteristics of Immortalized Human Renal Proximal Tubule Epithelial Cells about?
The proximal tubules, which are part of the kidney, maintain blood homeostasis by absorbing amino acids, glucose, water, and ions such as sodium (Na), potassium, and bicarbonate. Proximal tubule dysfunction is associated with the pathogenesis of many kidney diseases. Renal proximal tubular epithelial cells (RPTECs) are responsible for the main functions of the proximal tubules. Therefore, in vitro experiments using RPTECs would greatly enhance our understanding of nephron physiology and pathobiology. It is preferable to use immortalized cell lines, such as human kidney-2 (HK-2) cells, because they are derived from humans and maintain growth indefinitely. However, tissue-specific RPTEC phenotypes, including apical-basal polarization, are frequently lost in conventional two-dimensional culture methods in part due to microenvironmental deficiencies. To overcome this limitation, we developed a three-dimensional (3D) spheroid culture method for HK-2 cells using an extracellular matrix. HK-2 spheroids in 3D culture formed a tubule-like architecture with cellular polarity and showed markedly restored Na transport function. 3D culture of HK-2 cells also increased expression of kidney devel
Who reads Three-dimensional Spheroid Culture Induces Apical-basal Polarity and the Original Characteristics of Immortalized Human Renal Proximal Tubule Epithelial Cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ken Mizuguchi; Hiromasa Aoki; Mineyoshi Aoyama; Yohei Kawaguchi; Yuko Waguri-Nagaya; Nobuyuki Ohte; Kiyofumi Asai
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)