Can I read iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment on EtoBox?

iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment by A. Polrot; J. R. Kirby; F. J. Olorunniji; J. W. Birkett; G. P. Sharples is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment about?

Standard methods of microbial cultivation only enable the isolation of a fraction of the total environmental bacteria. Numerous techniques have been developed to increase the success of isolation and cultivation in the laboratory, some of which derive from diffusion chambers. In a diffusion chamber, environmental bacteria in agar medium are put back in the environment to grow as close to their natural conditions as possible, only separated from the environment by semi-permeable membranes. In this study, the iChip, a device that possesses hundreds of mini diffusion chambers, was used to isolate tributyltin (TBT) resistant and degrading bacteria. IChip was shown to be efficient at increasing the number of cultivable bacteria compared to standard methods. TBT-resistant strains belonging to Oceanisphaera sp., Pseudomonas sp., Bacillus sp. and Shewanella sp. were identified from Liverpool Dock sediment. Among the isolates in the present study, only members of Pseudomonas sp. were able to use TBT as a sole carbon source. It is the first time that members of the genus Oceanisphaera have been shown to be TBT-resistant. Although iChip has been used in the search for molecules of biomedical

Who reads iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment?

Author

A. Polrot; J. R. Kirby; F. J. Olorunniji; J. W. Birkett; G. P. Sharples

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)