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This open-end treatise on methods concerning pro tein separation had its beginning in an American Chemical Society symposium entitled'Con­ temporary Protein Separation Methods'which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles för achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaining insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g., chemically modified, deriving from a specific tissue, conjugated to different moieties, etc.) require meticulous trial and selection and/or modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides

Author

NICHOLAS CATSIMPOOLAS

Publisher

New York ; London: Plenum Press

Published

2013

Language

EN

ISBN

9781468499841

Subjects

Science, Chemistry, Biology