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Protocol to prepare mouse spinal cord for patch-clamp and histology experiments 2 Before you begin 2 Institutional permissions 2 Preparation of artificial cerebrospinal fluid (ACSF) solutions (for patch-clamp experiments) 2 Preparation of agarose block (for patch-clamp experiments) 3 VibroCheck and vibratome setting (for patch-clamp experiments) 3 Preparation of solutions (for histology experiments) 3 Key resources table 4 Materials and equipment 5 Lattice-shaped storage chamber 5 Step-by-step method details 6 Spinal cord preparation (for patch-clamp and histology experiments) 6 Making acute spinal cord slices with a vibratome (for patch-clamp experiments) 7 Preparation of spinal cord for cryosectioning (for histology experiments) 8 Antigen retrieval and immunofluorescence staining (for histology experiments) 10 Expected outcomes 11 Limitations 11 Troubleshooting 11 Problem 1 11 Potential solution 11 Problem 2 12 Potential solution 12 Problem 3 12 Potential solution 13 Problem 4 13 Potential solution 13 Problem 5 13 Potential solution 13 Resource availability 13 Lead contact 13 Materials availability 13 Data and code availability 13 Supplemental information 13 Acknowledgments 13 Au

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EN

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nonfiction