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Constructing Plant Radiation Hybrid Panels by Julie Wardrop; John Snape; Wayne Powell; Gordon C. Machray is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Constructing Plant Radiation Hybrid Panels about?

## Summary Radiation hybrid (RH) mapping, a somatic cell genetic technique, has been developed in animal systems as a general approach for the construction of long‐range physical maps of chromosomes. This statistical method relies on X‐ray induced breakage of chromosomes to determine the physical distance between markers, as well as their order on the chromosome. The method can be applied to single chromosomes or across the whole genome. The generation of plant (barley) radiation hybrids and their culture __in vitro__ is described here. PCR‐based marker systems are used to verify hybrid status and to demonstrate genome coverage. RH panels of the type generated can be used for physical mapping, map‐based cloning, or sequence contig assembly. RH resources will greatly aid the physical characterisation of crop plants with large genomes.

Who reads Constructing Plant Radiation Hybrid Panels?

Author

Julie Wardrop; John Snape; Wayne Powell; Gordon C. Machray

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2002

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)