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Can I read Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes on EtoBox?
Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes by Guy Bauw; Hanne Holm Rasmussen; Marc Van Den Bulcke; Prof. Jozef Van Damme; Magda Puype; Borbala Gesser; Julio E. Celis; Joël Vandekerckhove is a Engineering article available to read on EtoBox.
What is Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes about?
## Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes We have used two-dimensional gel electrophoresis as agenera1"preparative" method to purify proteins for microsequencing analysis. In the first experiments, proteins derived from a total extract of Nicotiana tabacum leaf tissue were directly blotted from the gel onto poly(4-vinyl-N-methylpyridinium iodide)-coated glass fiber sheets. The major spots were excised and subjected to NH2-terminal sequence analysis, which made it possible to identify five of the eight selected proteins, while two more were recognized by generated internal sequences. In a second set ofexperiments, proteins of human origin were separated on multiple two-dimensional gels and the Coomassie Brilliant Blue-stained spots were excised from the gels. The combined spots were re-eluted and concentrated in a new gel and blotted on Immobilon. They were fragmented by in situ proteolysis and the generated peptides were separated by reverse phase-high performance liquid chromatography and sequenced. At the average, the internal sequences that were obtained covered 35 residues per protein and allowe
Who reads Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Guy Bauw; Hanne Holm Rasmussen; Marc Van Den Bulcke; Prof. Jozef Van Damme; Magda Puype; Borbala Gesser; Julio E. Celis; Joël Vandekerckhove
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)
- Published
- 1990
- Language
- EN
- Field
- Engineering (Physical Sciences)