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Can I read Optogenetic manipulation of calcium signals in single T cells in vivo on EtoBox?
Optogenetic manipulation of calcium signals in single T cells in vivo by Bohineust, Armelle; Garcia, Zacarias; Corre, Béatrice; Lemaître, Fabrice; Bousso, Philippe is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Optogenetic manipulation of calcium signals in single T cells in vivo about?
By offering the possibility to manipulate cellular functions with spatiotemporal control, optogenetics represents an attractive tool for dissecting immune responses. However, applying these approaches to single cells in vivo remains particularly challenging for immune cells that are typically located in scattering tissues. Here, we introduce an improved calcium actuator with sensitivity allowing for two-photon photoactivation. Furthermore, we identify an actuator/reporter combination that permits the simultaneous manipulation and visualization of calcium signals in individual T cells in vivo. With this strategy, we document the consequences of defined patterns of calcium signals on T cell migration, adhesion, and chemokine release. Manipulation of individual immune cells in vivo should open new avenues for establishing the functional contribution of single immune cells engaged in complex reactions.
Who reads Optogenetic manipulation of calcium signals in single T cells in vivo?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Bohineust, Armelle; Garcia, Zacarias; Corre, Béatrice; Lemaître, Fabrice; Bousso, Philippe
- Publisher
- Nature Publishing Group; Springer Science and Business Media LLC; [London]: Nature Publishing Group, 2010-; Society for Mining, Metallurgy and Exploration Inc.; Research Square (ISSN 2041-1723)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)