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Microdissection molecular copy‐number counting (μMCC)—unlocking cancer archives with digital PCR by F McCaughan; E Darai‐Ramqvist; AT Bankier; BA Konfortov; N Foster; PJ George; TH Rabbitts; M Kost‐Alimova; PH Rabbitts; PH Dear is a Medicine article available to read on EtoBox.

What is Microdissection molecular copy‐number counting (μMCC)—unlocking cancer archives with digital PCR about?

## Abstract Most cancer genomes are characterized by the gain or loss of copies of some sequences through deletion, amplification or unbalanced translocations. Delineating and quantifying these changes is important in understanding the initiation and progression of cancer, in identifying novel therapeutic targets, and in the diagnosis and prognosis of individual patients. Conventional methods for measuring copy‐number are limited in their ability to analyse large numbers of loci, in their dynamic range and accuracy, or in their ability to analyse small or degraded samples. This latter limitation makes it difficult to access the wealth of fixed, archived material present in clinical collections, and also impairs our ability to analyse small numbers of selected cells from biopsies. Molecular copy‐number counting (MCC), a digital PCR technique, has been used to delineate a non‐reciprocal translocation using good quality DNA from a renal carcinoma cell line. We now demonstrate μMCC, an adaptation of MCC which allows the precise assessment of copy number variation over a significant dynamic range, in template DNA extracted from formalin‐fixed paraffin‐embedded clinical biopsies. Further

Who reads Microdissection molecular copy‐number counting (μMCC)—unlocking cancer archives with digital PCR?

Author

F McCaughan; E Darai‐Ramqvist; AT Bankier; BA Konfortov; N Foster; PJ George; TH Rabbitts; M Kost‐Alimova; PH Rabbitts; PH Dear

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

2008

Language

EN

Field

Medicine (Health Sciences)