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Linking autotrophic activity in environmental samples with specific bacterial taxa by detection of ^13^C‐labelled fatty acids by Claudia Knief; Karlheinz Altendorf; André Lipski is a Environmental Science article available to read on EtoBox.

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## Summary A method for the detection of physiologically active autotrophic bacteria in complex microbial communities was developed based on labelling with the stable isotope ^13^C. Labelling of autotrophic nitrifying, sulphur‐oxidizing and iron‐oxidizing populations was performed __in situ__ by incubation with NaH[^13^C]O~3~. Incorporated label into fatty acid methyl esters (FAMEs) was detected and quantified using gas chromatography‐mass spectrometry in single ion monitoring mode. Before the analyses of different environmental samples, the protocol was evaluated in pure culture experiments. In different environmental samples a selective labelling of fatty acids demonstrated which microbial taxa were responsible for the respective chemolithoautotrophic activity. The most strongly labelled fatty acids of a sample from a sulphide treating biofilter from an animal rendering plant were __cis__‐7‐hexadecenoic acid (16:1 cis7) and 11‐methyl hexadecanoic acid (16:0 11methyl), which are as‐yet not known for any sulphide‐oxidizing autotroph. The fatty acid labelling pattern of an experimental biotrickling filter sample supplied with dimethyl disulphide clearly indicated the presence and ac

Who reads Linking autotrophic activity in environmental samples with specific bacterial taxa by detection of ^13^C‐labelled fatty acids?

Author

Claudia Knief; Karlheinz Altendorf; André Lipski

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1462-2912)

Published

2003

Language

EN

Field

Environmental Science (Physical Sciences)