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Material surfaces affect the protein expression patterns of human macrophages: A proteomics approach by Donna Lee M. Dinnes; Helder Marçal; Stephen M. Mahler; J. Paul Santerre; Rosalind S. Labow is a Engineering article available to read on EtoBox.
What is Material surfaces affect the protein expression patterns of human macrophages: A proteomics approach about?
## Abstract Monocyte‐derived macrophages (MDM) are key inflammatory cells and are central to the foreign body response to implant materials. MDM have been shown to exhibit changes in actin cytoskeleton, multinucleation, cell size, and function in response to small alterations in polycarbonate‐urethane (PCNU) surface chemistry. Although PCNU chemistry has an influence on __de novo__ protein synthesis, no assessments of the protein expression profiles of MDM have yet been reported. The rapid emerging field of expression proteomics facilitates the study of changes in cellular protein profiles in response to their microenvironment. The current study applied proteomic techniques, 2‐dimensional electrophoresis (2‐DE) combined with MALDI‐ToF (matrix assisted laser desorption ionization–time of flight) mass spectrometry, to determine differences in MDM protein expression influenced by PCNU. Results indicated that MDM responded to material chemistry by modulation of structural proteins (i.e. actin, vimentin, and tubulin). Additionally, intracellular protein modulation which requires proteins responsible for trafficking (i.e. chaperone proteins) and protein structure modification (i.e. bond
Who reads Material surfaces affect the protein expression patterns of human macrophages: A proteomics approach?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Donna Lee M. Dinnes; Helder Marçal; Stephen M. Mahler; J. Paul Santerre; Rosalind S. Labow
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)
- Published
- 2006
- Language
- EN
- Field
- Engineering (Physical Sciences)