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Analysis of negatively ‘charge tagged’ DNA by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by Kurt Berlin; Ivo G. Gut is a Chemistry article available to read on EtoBox.

What is Analysis of negatively ‘charge tagged’ DNA by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry about?

An improvement in detectability and stability of DNA analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) using oligonucleotides modified with a neutralized backbone and a fixed single, positive charge was recently reported. The attachment of the positive charge requires a primary amino group, limiting this approach to accordingly functionalized DNA. The method described here uses backbone neutralized DNA for the same purpose, with a single unmodified phosphate in the DNA backbone carrying the negative charge. Thus, no chemical modification other than neutralizing the remaining charges on the phosphorothioates is required. This is performed in a single methylation step. The enhancement in sensitivity is comparable to that for DNA carrying a single positive charge, interestingly even when using the same non-protonating matrix. The mechanistic implications of these findings regarding the MALDI process are discussed. The DNA derivatization methods presented help to make MALDI-MS of DNA applicable and competitive for genome analysis and medical diagnostics.

Who reads Analysis of negatively ‘charge tagged’ DNA by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry?

Author

Kurt Berlin; Ivo G. Gut

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

1999

Language

EN

Field

Chemistry (Physical Sciences)