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Cold stress regulation of a bi-functional 3-dehydroquinate dehydratase/shikimate dehydrogenase (DHQ/SDH)-like gene in the freshwater green alga Spirogyra varians by Jong Won Han; Key Pyoung Lee; Minchul Yoon; Sung Ho Kang; Gwang Hoon Kim is a Earth and Planetary Sciences article available to read on EtoBox.

What is Cold stress regulation of a bi-functional 3-dehydroquinate dehydratase/shikimate dehydrogenase (DHQ/SDH)-like gene in the freshwater green alga Spirogyra varians about?

## Abstract A low temperature up-regulated gene was isolated from __Spirogyra varians__ using the differentially expressed gene method. The full cDNA sequence consisted of 1798 bp with an open reading frame of 1560 bp encoding 520 amino acid residues, which had neither a cleavage site nor a signal peptide. The deduced amino acid sequence was highly similar to the bi-functional 3-dehydroquinate dehydratase/shikimate dehydrogenase (DHQ/SDH) gene of higher plants. As the gene was cold regulated, we named it SVCR3 (__Spirogyra varians__ cold regulated gene). SVCR3 had two conserved domains of DHQase I and SDH (AroE), as well as a shikimate-binding site as found in other DHQ/SDH genes. Northern blot analysis showed that SVCR3 was up-regulated at 4°C. When combined with light (>50 μmol photon m^-2^ s^-1^) the expression of SVCR3 increased more at 4°C, but the expression was not affected by light intensity alone up to 200 μmol photon m^-2^ s^-1^ at temperatures higher than 10°C. Using the zymogram method (which is a visualization method for gel areas containing specific enzymes based on chromogenic reactions), we showed that there were three isozymes of DHQ/SDH in __Spirogyra varians__. T

Who reads Cold stress regulation of a bi-functional 3-dehydroquinate dehydratase/shikimate dehydrogenase (DHQ/SDH)-like gene in the freshwater green alga Spirogyra varians?

Author

Jong Won Han; Key Pyoung Lee; Minchul Yoon; Sung Ho Kang; Gwang Hoon Kim

Publisher

Walter de Gruyter GmbH

Published

2009

Field

Earth and Planetary Sciences (Physical Sciences)