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Rapid development in vitro and in vivo of resistance to ceftazidime in biofilm-growing Pseudomonas aeruginosa due to chromosomal β-lactamase by NIELS Bagge; OANA Ciofu; LENE T. Skovgaard; NIELS HØIby is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Rapid development in vitro and in vivo of resistance to ceftazidime in biofilm-growing Pseudomonas aeruginosa due to chromosomal β-lactamase about?

The aim of this study was to examine the development of resistance of biofilm‐growing __P. aeruginosa__ during treatment with ceftazidime. Biofilms were established in vitro using a modified Robbins device (MRD) and in vivo in the rat model of chronic lung infection. Three __P. aeruginosa__ strains isolated from the lungs of cystic fibrosis (CF) patients (MIC~ceftazidime~‐basal/induced β‐lactamase activity: PAO 579= 0.8 mg/l‐19/550 milliunits, 19676A=50 mg/l‐38/957 milliunits, 17107B=100 mg/l‐504/947 milliunits) were studied. After 1 or 2 weeks of continuous or intermittent (4 h/day) administration of ceftazidime to biofilms established in MDR, a statistically significant development of resistance to ceftazidime in PAO 579 or 19676A bacterial populations occurred. When ceftazidime was administered 4 h/day (200 mg/1) for 2 weeks, the frequency of resistant 19676A having MIC>25 mg/l was 4.4. 10^−1^compared to 6.0 · 10^−5^in the control biofilm. The same trend was observed after continuous administration of ceftazidime. MIC~ceftazidime~ of the more resistant variants was increased 500‐fold for PAO 579 and 8‐fold for 19676A, and the specific basal β‐lactamase activities from 19 to 1,40

Who reads Rapid development in vitro and in vivo of resistance to ceftazidime in biofilm-growing Pseudomonas aeruginosa due to chromosomal β-lactamase?

Author

NIELS Bagge; OANA Ciofu; LENE T. Skovgaard; NIELS HØIby

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0365-5555)

Published

2000

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)