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Can I read Developments toward a complete micro-total analysis system for Duchenne muscular dystrophy diagnosis on EtoBox?
Developments toward a complete micro-total analysis system for Duchenne muscular dystrophy diagnosis by Jerome P Ferrance; Qirong Wu; Braden Giordano; Casandra Hernandez; Yien Kwok; Karen Snow; Steve Thibodeau; James P Landers is a Chemistry article available to read on EtoBox.
What is Developments toward a complete micro-total analysis system for Duchenne muscular dystrophy diagnosis about?
The diagnosis of Duchenne muscular dystrophy (DMD) has historically utilized either PCR or requires Southern blot analysis, a southern blot analysis, however, is not amenable to incorporation in a microdevice format. A PCR amplification-based method has been developed, and we have previously coupled this amplification with microchip separation of the PCR fragments for DMD diagnosis. Diagnoses of affected patients were performed by comparing exon concentrations to those of control samples amplified at the same time. To accurately identify mutations in patient samples, this work established normal ranges for the concentration of each amplified exon fragment using control samples amplified over successive days. Our studies show that the number of cycles used in the amplification process affects this range. Affected patient samples were analyzed using these normal ranges and the mutations detected by Southern blot analysis were also diagnosed using the microchip separation method. Employing the microchip separation method decreases the time required for the analysis, but the time required for DNA purification and PCR amplification must also be decreased for faster total analysis of pat
Who reads Developments toward a complete micro-total analysis system for Duchenne muscular dystrophy diagnosis?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Jerome P Ferrance; Qirong Wu; Braden Giordano; Casandra Hernandez; Yien Kwok; Karen Snow; Steve Thibodeau; James P Landers
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)