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Electrophoresis-assisted open-tubular liquid chromatography/mass spectrometry for the analysis of lipooligosaccharide expressed by Campylobacter jejuni by Jianjun Li; Michiaki Koga; Denis Brochu; Nobuhiro Yuki; Kenneth Chan; Michel Gilbert is a Engineering article available to read on EtoBox.
What is Electrophoresis-assisted open-tubular liquid chromatography/mass spectrometry for the analysis of lipooligosaccharide expressed by Campylobacter jejuni about?
## Abstract Lipooligosaccharide (LOS) is the major component of the external membrane of __Campylobacter jejuni__. LOS contains a hydrophobic moiety, lipid A, and a hydrophilic moiety, oligosaccharide. Due to the unique mimicry of human ganglioside structures and potential involvement in the induction of the autoimmune polyneuropathies, Guillain–Barré and Miller Fisher syndromes, the structural characterization of __C. jejuni__ LOS has received much attention. We have been using capillary zone electrophoresis–mass spectrometry to analyze __O__‐deacylated LOS from __C. jejuni__. In an attempt to optimize the separation conditions, the effect of methanol on the separation of LOS was investigated. It was found that methanol resulted in stronger adsorption of LOS onto the wall of fused‐silica capillary. In this paper, we applied this adsorption to perform electrophoresis‐assisted open‐tubular liquid chromatography electrospray mass spectrometry for the analysis of __O__‐deacylated LOS mixtures from __C. jejuni__. The analytical potential of the proposed strategy for the analysis of __O__‐deacylated LOS glycoforms from five bacterial colonies is demonstrated. Online tandem mass spectrom
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- Author
- Jianjun Li; Michiaki Koga; Denis Brochu; Nobuhiro Yuki; Kenneth Chan; Michel Gilbert
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)
- Published
- 2005
- Language
- EN
- Field
- Engineering (Physical Sciences)