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Biuret Test Protocol for Protein Estimation by Kal Buenaflor is a document available to read on EtoBox.
What is Biuret Test Protocol for Protein Estimation about?
The document provides instructions for preparing a standard curve using the Biuret test to quantitatively estimate the amount of protein in an unknown sample. The Biuret test works by reacting peptide bonds in proteins with copper sulfate to form a violet colored complex. More peptide bonds yield greater color intensity that can be measured with a colorimeter. The procedure involves heating a protein solution with sodium hydroxide, adding Biuret reagent, incubating, and measuring optical density at 540nm to
- Author
- Kal Buenaflor
- Language
- EN