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Biscoclaurine alkaloid cepharanthine inhibits the growth of primary effusion lymphoma in vitro and in vivo and induces apoptosis via suppression of the NF-κB pathway by Naoko Takahashi-Makise; Shinya Suzu; Masateru Hiyoshi; Takeo Ohsugi; Harutaka Katano; Kazuo Umezawa; Seiji Okada is a Medicine article available to read on EtoBox.

## Abstract Primary effusion lymphoma (PEL) is a unique and recently identified non‐Hodgkin's lymphoma that was originally identified in patients with AIDS. PEL is caused by the Kaposi sarcoma‐associated herpes virus (KSHV/HHV‐8) and shows a peculiar presentation involving liquid growth in the serous body cavity and a poor prognosis. As the nuclear factor (NF)‐**κ**B pathway is activated in PEL and plays a central role in oncogenesis, we examined the effect of a biscoclaurine alkaloid, cepharanthine (CEP) on PEL derived cell lines (BCBL‐1, TY‐1 and RM‐P1), __in vitro__ and __in vivo__. An methylthiotetrazole assay revealed that the cell proliferation of PEL cell lines was significantly suppressed by the addition of CEP (1–10 μg/ml). CEP also inhibited NF‐**κ**B activation and induced apoptotic cell death in PEL cell lines. We established a PEL animal model by intraperitoneal injection of BCBL‐1, which led to the development of ascites and diffuse infiltration of organs, without obvious solid lymphoma formation, which resembles the diffuse nature of human PEL. Intraperitoneal administration of CEP inhibited ascites formation and diffuse infiltration of BCBL‐1 without significant sys

Author

Naoko Takahashi-Makise; Shinya Suzu; Masateru Hiyoshi; Takeo Ohsugi; Harutaka Katano; Kazuo Umezawa; Seiji Okada

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2009

Language

EN

Field

Medicine (Health Sciences)