Can I read DNA interstrand cross-links induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine on EtoBox?
DNA interstrand cross-links induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine by Rozelle, Aaron L. (author);Cheun, Young (author);Vilas, Caroline K. (author);Koag, Myong-Chul (author);Lee, Seongmin (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is DNA interstrand cross-links induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine about?
## Abstract Oxidative damage to DNA generates 7,8-dihydro-8-oxoguanine (oxoG) and 7,8-dihydro-8-oxoadenine (oxoA) as two major lesions. Despite the comparable prevalence of these lesions, the biological effects of oxoA remain poorly characterized. Here we report the discovery of a class of DNA interstrand cross-links (ICLs) involving oxidized nucleobases. Under oxidative conditions, oxoA, but not oxoG, readily reacts with an opposite base to produce ICLs, highlighting a latent alkylating nature of oxoA. Reactive halogen species, one-electron oxidants, and the myeloperoxidase/H~2~O~2~/Cl^−^ system induce oxoA ICLs, suggesting that oxoA-mediated cross-links may arise endogenously. Nucleobase analog studies suggest C2-oxoA is covalently linked to N2-guanine and N3-adenine for the oxoA-G and oxoA-A ICLs, respectively. The oxoA ICLs presumably form via the oxidative activation of oxoA followed by the nucleophilic attack by an opposite base. Our findings provide insights into oxoA-mediated mutagenesis and contribute towards investigations of oxidative stress-induced ICLs and oxoA-based latent alkylating agents.
Who reads DNA interstrand cross-links induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Rozelle, Aaron L. (author);Cheun, Young (author);Vilas, Caroline K. (author);Koag, Myong-Chul (author);Lee, Seongmin (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)