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Analysis of Protein Interactions on Protein Arrays by a Wavelength Interrogation-based Surface Plasmon Resonance Biosensor by Jong Seol Yuk; Se-Hui Jung; Jae-Wan Jung; Duk-Geun Hong; Jeong-A Han; Young-Myeong Kim; Kwon-Soo Ha is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Analysis of Protein Interactions on Protein Arrays by a Wavelength Interrogation-based Surface Plasmon Resonance Biosensor about?
## Abstract We have investigated whether surface plasmon resonance (SPR) sensors based on the wavelength interrogation are able to analyze protein interactions on protein arrays. The spectral SPR sensor was self‐constructed and its detection limit, expressed as the minimal refractive index variation, was calculated to be 6.6×10^−5^ with the signal fluctuation of 1.0×10^−5^. The protein array surface was modified by a mixed thiol monolayer to immobilize proteins. Protein arrays were analyzed by the line‐scanning mode of the SPR sensor, which scanned every 100 μm along the central line of array spots and the scanned results were presented by color spectra from blue to red. Glutathione __S__‐transferase (GST)‐rac1 caused a concentration‐dependent increase of SPR wavelength shift on protein arrays. The surface structure of the protein arrays was analyzed by atomic force microscopy. Specific interactions of antigens with antibodies were analyzed on the protein arrays by using three antibodies and eight proteins. These results suggest that the wavelength interrogation‐based SPR sensor can be used as the biosensor for the high‐throughput analysis of protein interactions on protein arrays.
Who reads Analysis of Protein Interactions on Protein Arrays by a Wavelength Interrogation-based Surface Plasmon Resonance Biosensor?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jong Seol Yuk; Se-Hui Jung; Jae-Wan Jung; Duk-Geun Hong; Jeong-A Han; Young-Myeong Kim; Kwon-Soo Ha
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)