About this Biochemistry, Genetics and Molecular Biology article

Single-cell Transcriptomic Analysis Uncovers Diverse and Dynamic Senescent Cell Populations by Noah Wechter; Martina Rossi; Carlos Anerillas; Dimitrios Tsitsipatis; Yulan Piao; Jinshui Fan; Jennifer L. Martindale; Supriyo De; Krystyna Mazan-Mamczarz; Myriam Gorospe is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

Senescence is a state of enduring growth arrest triggered by sublethal cell damage. Given that senescent cells actively secrete proinflammatory and matrix-remodeling proteins, their accumulation in tissues of older persons has been linked to many diseases of aging. Despite intense interest in identifying robust markers of senescence, the highly heterogeneous and dynamic nature of the senescent phenotype has made this task difficult. Here, we set out to comprehensively analyze the senescent transcriptome of human diploid fibroblasts at the individual-cell scale by performing single-cell RNA-sequencing analysis through two approaches. First, we characterized the different cell states in cultures undergoing senescence triggered by different stresses, and found distinct cell subpopulations that expressed mRNAs encoding proteins with roles in growth arrest, survival, and the secretory phenotype. Second, we characterized the dynamic changes in the transcriptomes of cells as they developed etoposide-induced senescence; by tracking cell transitions across this process, we found two different senescence programs that developed divergently, one in which cells expressed traditional senescence

Author

Noah Wechter; Martina Rossi; Carlos Anerillas; Dimitrios Tsitsipatis; Yulan Piao; Jinshui Fan; Jennifer L. Martindale; Supriyo De; Krystyna Mazan-Mamczarz; Myriam Gorospe

Publisher

Impact Journals, LLC

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)