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Measurement of Total Phospholipids in Urine of Patients Treated with Gentamicin by Darren A. Saunders; Evan J. Begg; Carl M. J. Kirkpatrick; James Yeo; Garry G. Graham; Ross R. Bailey is a Medicine article available to read on EtoBox.

What is Measurement of Total Phospholipids in Urine of Patients Treated with Gentamicin about?

__Aims__ The excretion of phospholipids in urine may be a marker of the early renal toxicity of the aminoglycoside antibiotics. Urinary phospholipids are formed in myeloid bodies which develop in the lysosomes of proximal tubules during treatment with the aminoglycosides, and overflow into the urine. __Methods__ Published assays were modified in order to measure the total phospholipid concentrations in human urine. Phospholipids were extracted from freeze‐dried urine samples, digested in concentrated sulphuric acid, and the inorganic phosphorus content determined by complexing with ammonium molybdate and measuring the absorbance at 820 nm. Ten septicaemic patients treated with gentamicin for 5–7 days had significantly higher urine phospholipid concentrations than 10 healthy untreated control subjects (__P__<0.0001). There was a negative linear relationship between phospholipid excretion and creatinine clearance (__r__ ^2^=0.71). __Results__ In 34 patients with acute pyelonephritis, increased phospholipid concentrations were observed prior to treatment compared with healthy controls (__P__<0.001) and did not alter during treatment with gentamicin. However, the phospholipid concentra

Who reads Measurement of Total Phospholipids in Urine of Patients Treated with Gentamicin?

Author

Darren A. Saunders; Evan J. Begg; Carl M. J. Kirkpatrick; James Yeo; Garry G. Graham; Ross R. Bailey

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley; Research Square (ISSN 0306-5251)

Published

1997

Language

EN

Field

Medicine (Health Sciences)