Can I read Cloning and Functional Expression of a Mouse Liver Organic Cation Transporter on EtoBox?

Cloning and Functional Expression of a Mouse Liver Organic Cation Transporter by Richard M. Green; Kathy Lo; Coreen Sterritt; David R. Beier is a Medicine article available to read on EtoBox.

What is Cloning and Functional Expression of a Mouse Liver Organic Cation Transporter about?

Hepatic uptake of organic cations is essential for the metabolism and secretion of numerous endobiotics and drugs. Several hepatic organic cation transporters have been kinetically defined, yet have not been isolated or cloned. We have isolated a complementary DNA (cDNA) from both murine liver and kidney cDNA libraries (mOct1/Slc22a1), and have functionally expressed it in Xenopus laevis oocytes. Although mOct1/Slc22a1 is homologous to previously cloned rat and human organic cation transporters, organic cation transport kinetics differed markedly. mOct1/Slc22a1-RNA injection of oocytes resulted in the saturable, time- and temperature-dependent uptake of the quaternary organic cation [14C]-tetraethylammonium ([14C]-TEA), with a Km of 38 micromol/L. TEA uptake was inhibited by several other organic cation drugs, but was not inhibited by the organic cation n-methyl-nicotinamide (NMN), being instead stimulated by it (fourfold). [14C]-TEA uptake was also stimulated by an inside-outside proton gradient. mOct1/Slc22a1-injected oocytes transported the organic cations [3H]-1-methyl-4-phenylpyridium and [3H]-choline chloride, but did not transport other classes of organic compounds. mOct1/Sl

Who reads Cloning and Functional Expression of a Mouse Liver Organic Cation Transporter?

Author

Richard M. Green; Kathy Lo; Coreen Sterritt; David R. Beier

Publisher

Ovid Technologies (Wolters Kluwer Health)

Published

1999

Language

EN

Field

Medicine (Health Sciences)