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Characterization of the glyoxalases of the malarial parasite Plasmodium falciparum and comparison with their human counterparts by Monique Akoachere; Rimma Iozef; Stefan Rahlfs; Marcel Deponte; Bengt Mannervik; Donald J. Creighton; Heiner Schirmer; Katja Becker is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract The glyoxalase system consisting of glyoxalase I (GloI) and glyoxalase II (GloII) constitutes a glutathione-dependent intracellular pathway converting toxic 2-oxoaldehydes, such as methylglyoxal, to the corresponding 2-hydroxyacids. Here we describe a complete glyoxalase system in the malarial parasite__Plasmodium falciparum__. The biochemical, kinetic and structural properties of cytosolic GloI (cGloI) and two GloIIs (cytosolic GloII named cGloII, and tGloII preceded by a targeting sequence) were directly compared with the respective isofunctional host enzymes. cGloI and cGloII exhibit lower__K__~m~values and higher catalytic efficiencies (__k__~cat~/__K__~m~) than the human counterparts, pointing to the importance of the system in malarial parasites. A Tyr185Phe mutant of cGloII shows a 2.5-fold increase in__K__~m~, proving the contribution of Tyr185 to substrate binding. Molecular models suggest very similar active sites/metal binding sites of parasite and host cell enzymes. However, a fourth protein, which has highest similarities to GloI, was found to be unique for malarial parasites; it is likely to act in the apicoplast, and has as yet undefined substrate specifi
Who reads Characterization of the glyoxalases of the malarial parasite Plasmodium falciparum and comparison with their human counterparts?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Monique Akoachere; Rimma Iozef; Stefan Rahlfs; Marcel Deponte; Bengt Mannervik; Donald J. Creighton; Heiner Schirmer; Katja Becker
- Publisher
- Walter de Gruyter GmbH
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)