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Alterations in the methylation status and expression of the raf oncogene in phenobarbital‐induced and spontaneous B6C3F1 mouse liver tumors by Jean S. Ray; Margaret L. Harbison; R. Michael McClain; Jay I. Goodman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Alterations in the methylation status and expression of the raf oncogene in phenobarbital‐induced and spontaneous B6C3F1 mouse liver tumors about?

## Abstract The liver tumor‐prone B6C3F1 mouse (C57BL/6 ♂ × C3H/He♀), in conjunction with the more susceptible C3H/He paternal strain and the resistant C57BL/6 maternal strain, is an excellent model for studying the mechanisms involved in carcinogenesis. The study reported here indicated that the B6C3F1 mouse inherited a maternal __raf__ allele containing a methylated site not present in the paternal allele. Seven days after partial hepatectomy or after administration of a promoting dose of phenobarbital (PB) for 14 d; __raf__ in B6C3F1 mouse liver was hypomethylated. The additional methylated site in the allele inherited from C57BL/6 was not maintained. The methylation status of __raf__ in the liver of the C57BL/6 mouse was not affected by PB treatment. This indicates that the B6C3F1 mouse is less capable of maintaining methylation of __raf__ than the C57BL/6 strain is. In both PB‐induced and spontaneous B6C3F1 liver tumors, __raf__ was hypomethylated in a nonrandom fashion. The level of __raf__ mRNA increased in seven of 10 PB‐induced tumors but in only one of five spontaneous tumors, whereas the level of Ha‐__ras__ mRNA increased in nine of 10 PB‐induced tumors and in four of fi

Who reads Alterations in the methylation status and expression of the raf oncogene in phenobarbital‐induced and spontaneous B6C3F1 mouse liver tumors?

Author

Jean S. Ray; Margaret L. Harbison; R. Michael McClain; Jay I. Goodman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)