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Studies of Apolipoprotein (a) Promoter from Subjects with Different Plasma Lipoprotein (a) Concentrations by June H. Wu; I-Neng Lee is a Medicine article available to read on EtoBox.

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## Objective: High plasma lipoprotein (a) [lp(a)] level is closely related to coronary heart disease and cerebral thrombosis. the lp(a) concentration is determined primarily by apolipoprotein (a) [apo(a)] gene and apo(a) mrna abundance has been found to vary with apo(a) isoform. our objective is to investigate whether apo(a) promoter activity is related to plasma lp(a) level. ## Design and methods: The 5' 1.4 kilobases (kb) promoter region of apo(a) was cloned into plasmid pgl-2 basic that carries luciferase reporter system. the promoter activity was assayed in hepg2 cells. dna sequence of the promoter was also determined. ## Results: Few nucleotide changes besides the variations at the five polymorphic sites: -1270 (tttta repeat), -868 (t repeat), -772 (a/g variation), +93 (c/t variation) and +121 (a/g variation) were observed in these promoters. apo(a) promoter activity differed in subjects with different plasma lp(a) levels. ## Conclusion: The sequence variation of apo(a) promoter region may contribute to the variation of its transcription activity.

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Author

June H. Wu; I-Neng Lee

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-9120)

Published

2003

Language

EN

Field

Medicine (Health Sciences)