Can I read Identification by Electrospray Tandem Mass Spectrometry of Spin-trapped Free Radicals from Oxidized 2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine on EtoBox?

Identification by Electrospray Tandem Mass Spectrometry of Spin-trapped Free Radicals from Oxidized 2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine by A. Reis; P. Domingues; A. J. V. Ferrer-Correia; M. R. M. Domingues is a Chemistry article available to read on EtoBox.

What is Identification by Electrospray Tandem Mass Spectrometry of Spin-trapped Free Radicals from Oxidized 2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine about?

## Abstract GPC radical species formed during oxidation of a glycerophosphocholine (16:0/18:1) under the Fenton reaction conditions were detected using a spin trap, 5,5‐dimethyl‐1‐pyrrolidine __N__‐oxide (DMPO). The stable spin‐trapped radical adducts were identified by mass spectrometry (MS) using electrospray (ES) as ionization method and characterized by tandem mass spectrometry (MS/MS). Radical adducts of oxidized free __sn__‐2 fatty acid and of oxidized intact GPC, containing one, two and three additional oxygen atoms, were assigned. DMPO adducts of oxidized intact GPC were observed as singly and doubly charged ions in ES‐MS, while adducts of oxidized free fatty acids were observed as singly charged ions. Oxidized free __sn__‐2 fatty acids and intact GPC‐DMPO adducts correspond to carbon‐ and oxygen‐centered radicals that were identified by MS/MS as alkyl, hydroxy‐alkyl, alkoxyl, hydroxy‐alkoxyl, peroxyl and hydroperoxide‐alkoxyl spin adducts. The DMPO molecule was attached predominantly at C~9~ of the oleic chain. The fragmentation pathway of spin adducts with two DMPO molecules strongly suggests the presence of species that were simultaneously carbon‐ and oxygen‐centered rad

Who reads Identification by Electrospray Tandem Mass Spectrometry of Spin-trapped Free Radicals from Oxidized 2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine?

Author

A. Reis; P. Domingues; A. J. V. Ferrer-Correia; M. R. M. Domingues

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2004

Language

EN

Field

Chemistry (Physical Sciences)