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Enrichment of Escherichia coli proteins by column chromatography on reactive dye columns by Rosslyn M. Birch; Conor O'Byrne; Ian R. Booth; Phillip Cash is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The reliable identification and analysis of the low abundance proteins expressed by a cell remains a key challenge in the study of cellular proteomes. The analysis of low abundance proteins is a particular problem when using two‐dimensional gel electrophoresis (2‐DE) to resolve the cellular proteins since the technology is unable to display the wide dynamic range of protein levels typically synthesized by cells. We have investigated the use of reactive dye compounds for the enrichment of low abundance cellular proteins prior to analysis by 2‐DE. The capacity of reactive dye compounds to bind specific protein species was used as the basis for a general chromatographic tool for protein enrichment. Six reactive dye compounds were investigated in detail for the analysis of __Escherichia coli__ proteins. Whole bacterial cell lysates were passed down columns prepared with the reactive dye compounds. The bound proteins were eluted with 1.5 M NaCl and analyzed by 2‐DE. Distinctive protein profiles were observed for the bound proteins recovered from the different reactive dye compounds. Selected proteins enriched by these methods were identified by peptide mass mapping. The enri

Who reads Enrichment of Escherichia coli proteins by column chromatography on reactive dye columns?

Author

Rosslyn M. Birch; Conor O'Byrne; Ian R. Booth; Phillip Cash

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)