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Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca by Johannes Wöstemeyer; Anke Burmester; Christoph Weigel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca about?

A plasmid (pAmN61) containing the NPT II structural gene (neomycin phosphotransferase) fused to the N-terminal region of a homologous actin gene was used for the transformation of Absidia glauca protoplasts. Neomycin resistant transformants could be selected for on complete medium containing 1.2 mg/ml neomycin sulfate. The physical presence of plasmid DNA in Absiclia glauca was demonstrated by retransformation into Escherichia coli and by Southern blot analysis. No integration of plasmid DNA at either one of the two actin loci was observed; Southern blot experiments provide evidence that pAmN61 is autonomously replicated in Absidia glauca.

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Author

Johannes Wöstemeyer; Anke Burmester; Christoph Weigel

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0172-8083)

Published

1987

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)