Can I read Liquid chromatography—mass spectrometry with on-line solid-phase extraction by a restricted-access C18 precolumn for direct plasma and urine injection on EtoBox?
Liquid chromatography—mass spectrometry with on-line solid-phase extraction by a restricted-access C18 precolumn for direct plasma and urine injection by R.A.M. van der Hoeven; A.J.P. Hofte; M. Frenay; H. Irth; U.R. Tjaden; J. van der Greef; A. Rudolphi; K.-S. Boos; G.Marko Varga; L.E. Edholm is a Chemistry article available to read on EtoBox.
What is Liquid chromatography—mass spectrometry with on-line solid-phase extraction by a restricted-access C18 precolumn for direct plasma and urine injection about?
In this paper, the on-line coupling of solid-phase extraction, based on a restricted-access support with liquid chromatography-mass spectrometry (LC-MS), for the analysis of biological samples is described. The system was tested with cortisol and prednisolone for plasma analysis and arachidonic acid lbr urine analysis. A precolumn packed with a 25-p.m C ~8 alkyl-diol support is used for direct plasma or urine injection. Using column-switching techniques, the analytes enriched on the precolumn are eluted to the analytical column without transfer loss. An on-line heart-cut technique was employed and only the analyte-containing fraction eluting from the LC column is directed to the MS to protect the LC-MS interface and ion-source from contamination. The whole system is operated in a parallel mode, that is, sample pre-treatment and LC-MS analysis are performed simultaneously to provide the shortest possible analysis time. The only off-line sample pre-treatment step required was centrifugation to remove particulate matter. With the fully automated system, total analysis times of 5 and 9.5 min were achieved for cortisol in serum and arachidonic acid in urine, respectively. Cortisol and r
Who reads Liquid chromatography—mass spectrometry with on-line solid-phase extraction by a restricted-access C18 precolumn for direct plasma and urine injection?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- R.A.M. van der Hoeven; A.J.P. Hofte; M. Frenay; H. Irth; U.R. Tjaden; J. van der Greef; A. Rudolphi; K.-S. Boos; G.Marko Varga; L.E. Edholm
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1997
- Language
- EN
- Field
- Chemistry (Physical Sciences)