Can I read Effect of Experimental Colchicine Encephalopathy on Brain Protein Synthesis and Tubulin Metabolism on EtoBox?

Effect of Experimental Colchicine Encephalopathy on Brain Protein Synthesis and Tubulin Metabolism by Donald N. McMartin; Lewis M. Schedlbauer is a Neuroscience article available to read on EtoBox.

What is Effect of Experimental Colchicine Encephalopathy on Brain Protein Synthesis and Tubulin Metabolism about?

## Abstract Colchicine blocks axoplasmic flow and produces neurofibrillary degeneration. Brain slices from mice injected intracerebrally with colchicine incorporated more [^14^C]leucine into protein and had a decreased uptake of [^14^C]leucine into the perchloric acid‐soluble pool than did their controls. Brain RNA content was decreased and free leucine increased by colchicine‐induced encephalopathy. The specific activities of proteins from subcellular fractions of colchicine‐injected brain were increased in the nuclear fraction, the 100,000‐__g__ supernatant, and its vinblastine‐precipitable tubulin. The ratio of the specific activity of the crude mitochondrial fraction to that of the total homogenate was decreased, as would consistent with impaired movement of newly labeled protein into synaptosomes. Colchicine‐injected brain extracts contained one or more cytosol fractions that stimulated ribosomal incorporation of [^14^C]leucine into protein in a cell‐free system. Colchicine‐binding‐activity measurements indicated loss of soluble and particulate tubulin in colchicine‐injected brains; the decrease of soluble tubulin was verified by its selective precipitation with vinblastine. C

Who reads Effect of Experimental Colchicine Encephalopathy on Brain Protein Synthesis and Tubulin Metabolism?

Author

Donald N. McMartin; Lewis M. Schedlbauer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3034)

Published

1978

Language

EN

Field

Neuroscience (Life Sciences)