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Stimulation of Plasma Membrane and Matrix Vesicle Enzyme Activity by Transforming Growth Factor-β in Osteosarcoma Cell Cultures by L. F. Bonewald; Z. Schwartz; L. D. Swain; V. Ramirez; J. Poser; B. D. Boyan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Stimulation of Plasma Membrane and Matrix Vesicle Enzyme Activity by Transforming Growth Factor-β in Osteosarcoma Cell Cultures about?

Transforming growth factor$ (TGFP) serves an important role in extracellular matrix formation by stimulating the production of numerous extracellular matrix proteins by connective tissue cells and by osteoblasts or bone-forming cells. TGFP has been shown to stimulate alkaline phosphatase (ALPase) activity in the rat osteoblast-like osteosarcoma cell line ROS 1712.8. Previous studies have shown that this enzyme is elevated during calcification of bone and that it is enriched in matrix vesicles, an extracellular organelle associated with initial hydroxyapatite formation. To test the hypothesis that TGFP plays a role in regulating mineral deposition in the matrix, the effects of TGFP on ALPase and phospholipase A, , two enzymes associated with mineralization, were examined. ROS 1712.8 cells were cultured at high and low density with recombinant human TGFP (0.1-10 ng/ml) to examine the influence of cell maturation on response to TGFP. Maximal stimulation of ALPase activity in the low density cultures was seen at 5 ng/ml; in high-density cultures, there was further stimulation at 10 ng/ml. There was a dose-dependent increase in ALPase activity seen in the matrix vesicles and plasma memb

Who reads Stimulation of Plasma Membrane and Matrix Vesicle Enzyme Activity by Transforming Growth Factor-β in Osteosarcoma Cell Cultures?

Author

L. F. Bonewald; Z. Schwartz; L. D. Swain; V. Ramirez; J. Poser; B. D. Boyan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)