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This document reports on the development of synthetic gene networks in Escherichia coli that function as cellular counters, capable of counting up to three induction events using two distinct designs: a riboregulated transcriptional cascade (RTC) and a DNA invertase cascade (DIC). The RTC counters utilize riboregulation to control gene expression in response to arabinose pulses, while the DIC counters employ recombinases to invert DNA segments for counting. Both designs demonstrate modularity and the abilit

Author

이원빈

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EN