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Can I read High-Throughput Investigation of Diverse Junction Elements in RNA Tertiary Folding on EtoBox?
High-Throughput Investigation of Diverse Junction Elements in RNA Tertiary Folding by Denny, Sarah Knight; Bisaria, Namita; Yesselman, Joseph David; Das, Rhiju; Herschlag, Daniel; Greenleaf, William James is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is High-Throughput Investigation of Diverse Junction Elements in RNA Tertiary Folding about?
RNAs fold into defined tertiary structures to function in critical biological processes. While quantitative models can predict RNA secondary structure stability, we are still unable to predict the thermodynamic stability of RNA tertiary structure. Here, we probe conformational preferences of diverse RNA two-way junctions to develop a predictive model for the formation of RNA tertiary structure. We quantitatively measured tertiary assembly energetics of >1,000 of RNA junctions inserted in multiple structural scaffolds to generate a "thermodynamic fingerprint" for each junction. Thermodynamic fingerprints enabled comparison of junction conformational preferences, revealing principles for how sequence influences 3-dimensional conformations. Utilizing fingerprints of junctions with known crystal structures, we generated ensembles for related junctions that predicted their thermodynamic effects on assembly formation. This work reveals sequence-structure-energetic relationships in RNA, demonstrates the capacity for diverse compensation strategies within tertiary structures, and provides a path to quantitative modeling of RNA folding energetics based on "ensemble modularity."
Who reads High-Throughput Investigation of Diverse Junction Elements in RNA Tertiary Folding?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Denny, Sarah Knight; Bisaria, Namita; Yesselman, Joseph David; Das, Rhiju; Herschlag, Daniel; Greenleaf, William James
- Publisher
- Elsevier Science; Elsevier ; Cell Press; Elsevier BV; Research Square (ISSN 0092-8674)
- Published
- 2018
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)