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Can I read Norovirus Detection in Water Samples at the Level of Single Virus Copies per Microliter Using a Smartphone-based Fluorescence Microscope on EtoBox?
Norovirus Detection in Water Samples at the Level of Single Virus Copies per Microliter Using a Smartphone-based Fluorescence Microscope by Soo Chung; Lane E. Breshears; Alana Gonzales; Christian M. Jennings; Christina M. Morrison; Walter Q. Betancourt; Kelly A. Reynolds; Jeong-Yeol Yoon is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Norovirus Detection in Water Samples at the Level of Single Virus Copies per Microliter Using a Smartphone-based Fluorescence Microscope about?
Norovirus is a widespread public health threat and has a very low infectious dose. This protocol presents the extremely sensitive mobile detection of norovirus from water samples using a custom-built smartphone-based fluorescence microscope and a paper microfluidic chip. Antibody-conjugated fluorescent particles are immunoagglutinated and spread over the paper microfluidic chip by capillary action for individual counting using a smartphone-based fluorescence microscope. Smartphone images are analyzed using intensity- and size-based thresholding for the elimination of background noise and autofluorescence as well as for the isolation of immunoagglutinated particles. The resulting pixel counts of particles are correlated with the norovirus concentration of the tested sample. This protocol provides detailed guidelines for the construction and optimization of the smartphone- and paper-based assay. In addition, a 3D-printed enclosure is presented to incorporate all components in a dark environment. On-chip concentration and the assay of higher concentrations are presented to further broaden the assay range. This method is the first to be presented as a highly sensitive mobile platform f
Who reads Norovirus Detection in Water Samples at the Level of Single Virus Copies per Microliter Using a Smartphone-based Fluorescence Microscope?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Soo Chung; Lane E. Breshears; Alana Gonzales; Christian M. Jennings; Christina M. Morrison; Walter Q. Betancourt; Kelly A. Reynolds; Jeong-Yeol Yoon
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)