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Altered Expression of Genes Regulating Angiogenesis in Experimental Androgen-independent Prostate Cancer by Heléne Gustavsson; Karin Jennbacken; Karin Welén; Jan-Erik Damber is a Medicine article available to read on EtoBox.

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## Abstract ## BACKGROUND The aim of this study was to investigate how the expression of genes regulating angiogenesis is altered when prostate cancer cells progress into androgen‐independency. ## METHODS A gene array specific for angiogenesis was used to compare the human prostate cancer cell line LNCaP (androgen‐dependent) with its more angiogenic and tumorigenic subline LNCaP‐19 (androgen‐independent). Results were verified with real‐time RT‐PCR, and further investigations were focused on the angiogenesis inhibitor a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1). Expression of ADAMTS1 was investigated in vitro as well as in subcutaneous tumors with real‐time RT‐PCR and Western blotting. Microvessel density (MVD), versican proteolysis and protein levels of TIMP‐2 and TIMP‐3, known as ADAMTS1 inhibitors, were also analyzed in tumor xenografts. ## RESULTS The gene array revealed decreased expression of ADAMTS1, ephrin‐A5, fibronectin 1, and neuropilin 1 in LNCaP‐19 compared to LNCaP, while expression of midkine and VEGF were increased. Further studies showed that mRNA and protein levels of ADAMTS1 were significantly lower in LNCaP‐19 compared to LNCaP, bo

Who reads Altered Expression of Genes Regulating Angiogenesis in Experimental Androgen-independent Prostate Cancer?

Author

Heléne Gustavsson; Karin Jennbacken; Karin Welén; Jan-Erik Damber

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)

Published

2007

Language

EN

Field

Medicine (Health Sciences)