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Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/Î2-catenin axis by Huang, Lifa; Li, Xu; Ye, Hui; Liu, Yajun; Liang, Xiaolong; Yang, Chao; Hua, Lin; Yan, Zhaoxian; Zhang, Xin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background Glioma is a common brain malignancy with high mortality. The competing endogenous RNA (ceRNA) networks may play key roles in cancer progression. This study was conducted to probe the role of long noncoding RNA (lncRNA) NCK1-AS1 in glioma progression and the involved mechanisms. ## Methods Microarray analyses were performed to explore the lncRNAs/miRNAs/genes with differential expression in glioma. NCK1-AS1 levels in glioma tissues and normal brain tissues, and in glioma cell lines and normal human glial cells were identified. The interactions among NCK1-AS1, miR-138-2-3p and TRIM24 were validated through luciferase reporter, RNA immunoprecipitation and RNA pull-down assays. Gain- and loss-of functions of NCK1-AS1, miR-138-2-3p and TRIM24 were performed to identify their roles in the behaviors of glioma cells. The activity of the Wnt/β-catenin pathway was measured. In vivo experiments were performed as well. ## Results High expression of NCK1-AS1 was found in glioma tissues and cells, especially in U251 cells. Online predictions and the integrated experiments identified that NCK1-AS1 elevated the TRIM24 expression through sponging miR-138-2-3p, and further activated th

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Author

Huang, Lifa; Li, Xu; Ye, Hui; Liu, Yajun; Liang, Xiaolong; Yang, Chao; Hua, Lin; Yan, Zhaoxian; Zhang, Xin

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2008-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1756-9966)

Published

2020

Language

EN

ISBN

9781304602008

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)