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Ex vivo formation of gastric metabolites of clenbuterol: Preliminary characterisation of their chemical structure by Gianfranco Brambilla; Simone di Bez; Donatella Pietraforte; Maurizio Minetti; Luigi Campanella; Alberto Loizzo is a Chemistry article available to read on EtoBox.
What is Ex vivo formation of gastric metabolites of clenbuterol: Preliminary characterisation of their chemical structure about?
The epidemiology of clenbuterol food-borne intoxication outbreaks indicates a possible discrepancy between the severity and long duration of clinical symptoms, and the presumed dose ingested as parent compound residue. In this work, we explore the possibility that clenbuterol could undergo to a biological transformation, in presence of salivary nitrites, at gastric pH (<3). Human salivary specimens were drawn before and after meal, accounting for the different physiological nitrite content (40 and 400 mol L -1 , respectively, as average). Clenbuterol (10 mol L -1 ) was then incubated within the pH range 2-6 and possible products monitored by liquid chromatography-mass spectrometry (LC-MS), drawing at regular intervals serial aliquots of the incubation mixture. With respect to controls, two differential peaks were noted along with a quantitative biotransformation of the parent compound, at pH values ≤3. Under pre-meal conditions, a 4 mono-nitro compound was identified as main metabolite, whereas under post-meal condition a second metabolite, showing a complete de-chlorination, along with the probable presence of three nitro groups on the aromatic ring, was revealed. The reaction was
Who reads Ex vivo formation of gastric metabolites of clenbuterol: Preliminary characterisation of their chemical structure?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Gianfranco Brambilla; Simone di Bez; Donatella Pietraforte; Maurizio Minetti; Luigi Campanella; Alberto Loizzo
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)