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Cellular Ubiquitination and Proteasomal Functions Positively Modulate Mammalian Nucleotide Excision Repair by Qi‐En Wang; Manzoor A. Wani; Jianming Chen; Qianzheng Zhu; Gulzar Wani; Mohamed A. El‐Mahdy; Altaf A. Wani is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The ubiquitin‐proteasome pathway is fundamental to synchronized continuation of many cellular processes, for example, cell‐cycle progression, stress response, and cell differentiation. Recent studies have shown that the ubiquitin‐proteasome pathway functions in the regulation of nucleotide excision repair (NER) in yeast. In order to investigate the role of the ubiquitin‐proteasome pathway in the NER of mammalian cells, global genomic repair (GGR), and transcription‐coupled repair (TCR) were examined in a mouse ts20 cell line that harbors a temperature‐sensitive ubiquitin‐activating enzyme (E1). We found that E1 inactivation‐induced ubiquitination deficiency decreased both GGR and TCR, indicating that the ubiquitination system is involved in the optimization of entire NER machinery in mammalian cells. We specifically inhibited the function of 19S proteasome subunit by overexpressing 19S regulatory complex hSug1 or its mutant protein hSug1mk in repair competent human fibroblast, OSU‐2, cells and compared their capacity for NER. The results showed that 19S regulatory complex positively modulates NER in cells. In addition, we treated OSU‐2 cells with the inhibitors of 20S s

Who reads Cellular Ubiquitination and Proteasomal Functions Positively Modulate Mammalian Nucleotide Excision Repair?

Author

Qi‐En Wang; Manzoor A. Wani; Jianming Chen; Qianzheng Zhu; Gulzar Wani; Mohamed A. El‐Mahdy; Altaf A. Wani

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)