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Distribution and Possible Role of Perinuclear Theca Proteins During Bovine Spermiogenesis by Dr. R. Oko; D. Maravei is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The perinuclear theca (PT) is a unique cytoskeletal element that encapsulates the mammalian sperm nucleus. It is divided into subacrosomal and postacrosomal regions. The objective of this study was to analyze and stage the intracellular distribution of several promiment bull PT proteins during spermatogenesis. For this purpose, polyclonal antibodies raised and affinitypurified against the 15.5‐, 25‐, 28‐, 32‐, 36‐, and 60‐kDa bull PT polypeptides were used as probes on sections of aldehyde‐fixed testes. Immunoperoxidase staining revealed that the PT polypeptides first appeared early in spermiogenesis, concomitant with early steps of development of the acrosomic system. Immunogold labeling further showed that these polypeptides were peripherally associated with the entire acrosomal membrane, before and during the attachment of the acrosomic vesicle onto the spermatid's nucleus. Once the acrosome had capped the nucleus the labeling resided mainly in the subacrosomal region of the spermatid, between the inner acrosomal membrane and nuclear envelope. Later, during the elongation of the spermatid's nucleus, the labeling with all antibodies except the anti‐15.5‐kDa antibody e

Who reads Distribution and Possible Role of Perinuclear Theca Proteins During Bovine Spermiogenesis?

Author

Dr. R. Oko; D. Maravei

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1059-910X)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)