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Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1→5)-α-l-Arabinan in the Pericarp of Cnr , a Ripening Mutant of Tomato by Caroline Orfila; Graham B. Seymour; William G.T. Willats; I. Max Huxham; Michael C. Jarvis; Colin J. Dover; Andrew J. Thompson; J. Paul Knox is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1→5)-α-l-Arabinan in the Pericarp of Cnr , a Ripening Mutant of Tomato about?

Cnr (colorless non-ripening) is a pleiotropic tomato (Lycopersicon esculentum) fruit ripening mutant with altered tissue properties including weaker cell-to-cell contacts in the pericarp (A.J. Thompson, M. Tor, C.S. Barry, J. Vrebalov, C. Orfila, M.C. Jarvis, J.J. Giovannoni, D. Grierson, G.B. Seymour [1999] Plant Physiol 120: 383-390). Whereas the genetic basis of the Cnr mutation is being identified by molecular analyses, here we report the identification of cell biological factors underlying the Cnr texture phenotype. In comparison with wild type, ripe-stage Cnr fruits have stronger, non-swollen cell walls (CW) throughout the pericarp and extensive intercellular space in the inner pericarp. Using electron energy loss spectroscopy imaging of calcium-binding capacity and anti-homogalacturonan (HG) antibody probes (PAM1 and JIM5) we demonstrate that maturation processes involving middle lamella HG are altered in Cnr fruit, resulting in the absence or a low level of HG-/calcium-based cell adhesion. We also demonstrate that the deposition of (1-->5)-alpha-L-arabinan is disrupted in Cnr pericarp CW and that this disruption occurs prior to fruit ripening. The relationship between the d

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Author

Caroline Orfila; Graham B. Seymour; William G.T. Willats; I. Max Huxham; Michael C. Jarvis; Colin J. Dover; Andrew J. Thompson; J. Paul Knox

Publisher

Oxford University Press (OUP)

Published

2001

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)