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Comparison of Two in Vitro Dendritic Cell Maturation Models for Screening Contact Sensitizers Using a Panel of Methacrylates by T. Rustemeyer; M. Preuss; B. M. E. Von Blomberg; P. K. Das; R. J. Scheper is a Medicine article available to read on EtoBox.

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**Abstract:** Allergen‐induced emigration and maturation of dendritic cells (DC) are pivotal steps in sparking off allergic contact dermatitis. __In vitro__ models, reflecting these steps, may provide tools for assessment of sensitizing capacities of putative contact allergens. Here, we evaluated the applicability of such models for a panel of methacrylate congeners, the sensitizing properties of which were established previously in clinical and experimental animal studies. First, using interleukin‐4 (IL‐4)/granulocyte–macrophage colony‐stimulating factor (GM‐CSF)‐induced, blood monocyte‐derived DC, hapten‐induced up‐regulation of maturation/activation markers, including CD80, CD83, CD86, chemokine receptors CXCR4 and CCR5, as well as the drug resistance related molecules __P__‐glycoprotein (Pgp) and lung resistance protein (LRP), were monitored by flow cytometry. Of note, whereas CD86 and CXCR4 were most sensitive in discriminating between the contact sensitizers and irritants included in the panel, i.e. sodium dodecyl sulphate (SDS) and croton oil (CO), assessment of CD83 and LRP expression reflected the relatively lower sensitizing capacity of methyl methacrylate. Second, using

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Author

T. Rustemeyer; M. Preuss; B. M. E. Von Blomberg; P. K. Das; R. J. Scheper

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0906-6705)

Published

2003

Language

EN

Field

Medicine (Health Sciences)