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Plasmid DNA Purification Methods Explained by Georgia Tsikala is a document available to read on EtoBox.
Plasmid DNA must be separated from host bacterial DNA and RNA. Several methods have been developed to create a cleared lysate that removes contaminants while leaving plasmid DNA soluble. Popular methods include SDS-alkaline denaturation, salt-SDS precipitation, and rapid boiling. The SDS-alkaline denaturation technique exploits differences in how plasmid and chromosomal DNA renature to leave plasmid DNA soluble while precipitating out contaminants.
- Author
- Georgia Tsikala
- Language
- EN